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Disk quotas in Windows 2000 are based on file ownership, which can change when moving folders from one server to another. Verify the owner of the newly-moved folders on the file/folder properties tab, and have the appropriate users take ownership of their files if necessary. In the meantime, MS Knowledge Base article 183322 offers a step-by-step explanation on enabling disk quotas.
Dig Deeper on Microsoft Windows 2000 Server Administration
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