If you are using NT 4.0 server to manage your printer and you have Windows 2000 Professional as a client, you lose...
the printer connection when you log off.
The Microsoft solution is to re-install each time you logon. The following batch file placed in your Startup folder is a more viable solution as it re-connects the printer each time you logon without any user intervention.
net use "NTSERVER" "Printer Name" Username /user:password
net use wo281117proom225 guest /user:guest